Seasonal Variation in Plant Polyphenols and Related Bioactivities across Three Years in Ten Tree Species as Visualized by Mass Spectrometric Fingerprint Mapping

The currently changing climates and environments place plants under many types of stresses that affect both their survival levels chemical defenses. gradual induction defenses in stressed plant populations could be monitored on a yearly basis unless seasonal variation natural defense obscures such monitoring schemes. Here, we studied the stability species-specific polyphenol composition content 10 tree species over three growing seasons using five replicate trees per species. We specifically measured hydrolyzable tannins (galloyl hexahydroxydiphenoyl derivatives), proanthocyanidins (procyanidins prodelphinidins), flavonols (kaempferol, quercetin kaempferol derivatives) quinic acid derivatives with group-specific UHPLC-DAD-MS/MS tool, together two bioactivities, protein precipitation capacity oxidative activity. With help fingerprint mapping found out differed lot between-year polyphenols was also partially specific to compound groups. Especially ellagitannins tended have declining patterns while opposite true for proanthocyanidins. Some showed minimal all variables, others even induced certain groups during 3-year study. For every species, either baseline qualitative quantitative chemistry or most plasticity production. used tools thus form good combination future studies attempting monitor overall changes due various biotic abiotic stress factors more controlled environments.